By M. Ahearne, Alicia J. El Haj, S. Rauz (auth.), Alicia El Haj, Dan Bader (eds.)
This quantity provides chosen peer-reviewed papers of the eighth overseas convention on phone & Stem phone Engineering (ICCM) 2010 in Dublin. The contributions are written by means of major scientists in mobile and Stem cellphone Engineering and the themes of the papers contain:
Computational phone Mechanics
Experimental innovations in cellphone Mechanics
Molecular and mobilephone Imaging
Cell Matrix Interactions
Mechanotransduction and cellphone mechanics
Stem cellphone area of interest
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Additional resources for 8th International Conference on Cell & Stem Cell Engineering (ICCE)
5% FBS supplemented with 100µg/ml dextran sulfate on the following day for 3 days. 5% sodium deoxycholate to obtain cell-free Matrix-A (3 washes) or Matrix B (6 washes). MSCs (Cambrex, passage 2) were cultured in low glucose Dulbecco’s modified Eagle’s medium (Gibco) and 10% FBS. Cells were maintained at subconfluency prior to being passaged 1:4 using TrypLE™ Express (Gibco). A. El Haj and D. ): 8th International Conference on Cell & Stem Cell Engineering, IFMBE Proceedings 30, pp. 31–34, 2011.
Isolated porcine chondrocytes were suspended in 2% agarose. Microchanneled and solid construct cylinders (Ø 6 x 4 mm) were fabricated and maintained in supplemented media containing TGF-β3 (10ηg/ml). Loaded solid and channeled constructs were subjected to a compressive strain amplitude of 10%, for 2 hours/day, 5 days/week, for a duration of 6 weeks. In the presence of TGF-β3, dynamic compressive loading in solid constructs resulted in an increase in sulphated glycosaminoglycan (sGAG) accumulation.
Electrophysiological Experiments For the experiments, testing the acute effect of OXA on the network activity, neurons were cultured on multi electrode arrays. These are glass culture chambers with 60 electrodes (diameter of 10 or 30 μm) incorporated in the bottom. In all experiments, cultures were moved to the measurement set up one hour prior to recording to adapt to the conditions and we recorded baseline activity. Then we replaced 300 μl of the medium by 300 μl of fresh medium as a control for the effect of administration of an equal amount of OXA containing medium.
8th International Conference on Cell & Stem Cell Engineering (ICCE) by M. Ahearne, Alicia J. El Haj, S. Rauz (auth.), Alicia El Haj, Dan Bader (eds.)